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The LAMP primer used in the present study.

Journal: Frontiers in Plant Science

Article Title: Rapid and accurate detection of Fusarium oxysporum f. sp. Lycopersici using one-pot, one-step LAMP-CRISPR/Cas12b method

doi: 10.3389/fpls.2024.1485884

Figure Lengend Snippet: The LAMP primer used in the present study.

Article Snippet: According to the conserved region of Fol gene SIX1 (GenBank: U66901.1), the LAMP primer was designed by using the Online NEB LAMP primer design tool ( https://lamp.neb.com/#!/ ).

Techniques: Sequencing

Selection of the LAMP primers for Fol one-step LAMP CRISPR/Cas12b detection system. The selection results of sixteen groups LAMP primer were shown from (A–P) respectively. Fol_SIXI_LAMP5 (E) exhibited a better performance than that of others.

Journal: Frontiers in Plant Science

Article Title: Rapid and accurate detection of Fusarium oxysporum f. sp. Lycopersici using one-pot, one-step LAMP-CRISPR/Cas12b method

doi: 10.3389/fpls.2024.1485884

Figure Lengend Snippet: Selection of the LAMP primers for Fol one-step LAMP CRISPR/Cas12b detection system. The selection results of sixteen groups LAMP primer were shown from (A–P) respectively. Fol_SIXI_LAMP5 (E) exhibited a better performance than that of others.

Article Snippet: According to the conserved region of Fol gene SIX1 (GenBank: U66901.1), the LAMP primer was designed by using the Online NEB LAMP primer design tool ( https://lamp.neb.com/#!/ ).

Techniques: Selection, CRISPR

Validation of the practical samples. Representative images of Fol-infected AMK for 5 months from (A) Pot cultivation experiments and (B) field experiments. (C) Amplification profiles of SIX1 of the practical samples from Pot cultivation experiments and field experiments by using Fol one-step LAMP-CRISPR/Cas12b method, the. The recombinant plasmid was served as the positive control (PC), while the nuclease-free water used for NTC. (D) Amplification profiles of SIX1 of the practical samples from Pot cultivation experiments and field experiments by using qPCR assays. (E) Anneal derivative of SIX1 amplicons within qPCR assays, with an average anneal derivative of 78°C.

Journal: Frontiers in Plant Science

Article Title: Rapid and accurate detection of Fusarium oxysporum f. sp. Lycopersici using one-pot, one-step LAMP-CRISPR/Cas12b method

doi: 10.3389/fpls.2024.1485884

Figure Lengend Snippet: Validation of the practical samples. Representative images of Fol-infected AMK for 5 months from (A) Pot cultivation experiments and (B) field experiments. (C) Amplification profiles of SIX1 of the practical samples from Pot cultivation experiments and field experiments by using Fol one-step LAMP-CRISPR/Cas12b method, the. The recombinant plasmid was served as the positive control (PC), while the nuclease-free water used for NTC. (D) Amplification profiles of SIX1 of the practical samples from Pot cultivation experiments and field experiments by using qPCR assays. (E) Anneal derivative of SIX1 amplicons within qPCR assays, with an average anneal derivative of 78°C.

Article Snippet: According to the conserved region of Fol gene SIX1 (GenBank: U66901.1), the LAMP primer was designed by using the Online NEB LAMP primer design tool ( https://lamp.neb.com/#!/ ).

Techniques: Infection, Amplification, CRISPR, Recombinant, Plasmid Preparation, Positive Control